Detailed information of TA system TA099632

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2767927..2768725
• Replicon: chromosome
• Accession: NZ_CP027586
• Organism: Escherichia coli strain 2012EL-2448

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0P7PH46
• Locus tag: C7A04_RS14765
• Protein ID: WP_000854896.1
• Coordinates: 2767927..2768304 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A234XUW5
• Locus tag: C7A04_RS14770
• Protein ID: WP_024175300.1
• Coordinates: 2768351..2768725 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A04_RS14730	2763204..2764742	-	1539	WP_001187182.1	lysine decarboxylation/transport transcriptional activator CadC	-
C7A04_RS14740	2765624..2765950	-	327	WP_000779483.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C7A04_RS14745	2765947..2766210	-	264	WP_001143297.1	type II toxin-antitoxin system ParD family antitoxin	-
C7A04_RS14750	2766282..2767148	-	867	WP_106913398.1	DUF4942 domain-containing protein	-
C7A04_RS14755	2767233..2767430	-	198	WP_000839256.1	DUF957 domain-containing protein	-
C7A04_RS14760	2767442..2767930	-	489	WP_000761662.1	hypothetical protein	-
C7A04_RS14765	2767927..2768304	-	378	WP_000854896.1	TA system toxin CbtA family protein	Toxin
C7A04_RS14770	2768351..2768725	-	375	WP_024175300.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C7A04_RS14775	2768888..2769109	-	222	WP_000692358.1	DUF987 domain-containing protein	-
C7A04_RS14780	2769172..2769648	-	477	WP_001186727.1	RadC family protein	-
C7A04_RS14785	2769664..2770149	-	486	WP_000214386.1	antirestriction protein	-
C7A04_RS14790	2770239..2771057	-	819	WP_001234751.1	DUF945 domain-containing protein	-
C7A04_RS14795	2771148..2771381	-	234	WP_001117568.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14210.19 Da
• Isoelectric Point: 7.2920

>T99632 WP_000854896.1 NZ_CP027586:c2768304-2767927 [Escherichia coli]
MKTLSDTHVREASRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T99632 NZ_CP027586:c2768304-2767927 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGCATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATAACATTACCCAGGGTAAGCATCCGGAGGCGCAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13664.42 Da
• Isoelectric Point: 6.4757

>AT99632 WP_024175300.1 NZ_CP027586:c2768725-2768351 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVKPCFGARLVQEGNRLHYLADRAGISGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHSVTLYAKGLTCEADTLGSCGYVYLTVYPTPATATTS

Nucleotide

• Length: 375 bp

>AT99632 NZ_CP027586:c2768725-2768351 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAA
ACCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGCGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATTCCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTGTTTATCCGACACCGGCAACCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A0P7PH46

Antitoxin

• AlphaFold DB: A0A234XUW5