Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1328605..1329259 Replicon chromosome
Accession NZ_CP027586
Organism Escherichia coli strain 2012EL-2448

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C7A04_RS07180 Protein ID WP_000244777.1
Coordinates 1328605..1329012 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C7A04_RS07185 Protein ID WP_000354046.1
Coordinates 1328993..1329259 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A04_RS07160 1324562..1326295 - 1734 WP_000813168.1 single-stranded-DNA-specific exonuclease RecJ -
C7A04_RS07165 1326301..1327011 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C7A04_RS07170 1327036..1327932 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C7A04_RS07175 1328044..1328565 + 522 WP_001055874.1 flavodoxin FldB -
C7A04_RS07180 1328605..1329012 - 408 WP_000244777.1 toxin CptA Toxin
C7A04_RS07185 1328993..1329259 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C7A04_RS07190 1329502..1330482 + 981 WP_000886063.1 tRNA-modifying protein YgfZ -
C7A04_RS07195 1330678..1331337 - 660 WP_000250274.1 hemolysin III family protein -
C7A04_RS07200 1331501..1331812 - 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
C7A04_RS07205 1331857..1333290 + 1434 WP_024178269.1 6-phospho-beta-glucosidase BglA -
C7A04_RS07210 1333347..1334090 - 744 WP_024178268.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T99627 WP_000244777.1 NZ_CP027586:c1329012-1328605 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T99627 NZ_CP027586:c1329012-1328605 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT99627 WP_000354046.1 NZ_CP027586:c1329259-1328993 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT99627 NZ_CP027586:c1329259-1328993 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References