Detailed information of TA system TA099625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 844241..844866
• Replicon: chromosome
• Accession: NZ_CP027586
• Organism: Escherichia coli strain 2012EL-2448

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C7A04_RS04605
• Protein ID: WP_000911330.1
• Coordinates: 844241..844639 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C7A04_RS04610
• Protein ID: WP_000450524.1
• Coordinates: 844639..844866 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A04_RS04585	840119..840319	+	201	WP_000383836.1	YpfN family protein	-
C7A04_RS04590	840429..841127	-	699	WP_000679812.1	esterase	-
C7A04_RS04595	841201..843216	-	2016	WP_000829394.1	tRNA cytosine(34) acetyltransferase TmcA	-
C7A04_RS04600	843231..844094	-	864	Protein_840	zinc metallopeptidase	-
C7A04_RS04605	844241..844639	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C7A04_RS04610	844639..844866	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C7A04_RS04615	845020..845733	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C7A04_RS04620	845946..846980	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
C7A04_RS04625	846997..847875	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C7A04_RS04630	848021..848593	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C7A04_RS04635	848593..849063	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T99625 WP_000911330.1 NZ_CP027586:c844639-844241 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T99625 NZ_CP027586:c844639-844241 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT99625 WP_000450524.1 NZ_CP027586:c844866-844639 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT99625 NZ_CP027586:c844866-844639 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33