Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 71242..71767 Replicon plasmid unnamed
Accession NZ_CP027585
Organism Escherichia coli strain 00-3076

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C7A02_RS26450 Protein ID WP_001159871.1
Coordinates 71462..71767 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag C7A02_RS26445 Protein ID WP_000813639.1
Coordinates 71242..71460 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A02_RS27445 67201..67412 + 212 Protein_57 hypothetical protein -
C7A02_RS27450 67416..67526 + 111 Protein_58 IS481 family transposase -
C7A02_RS26415 67803..68534 + 732 WP_000850413.1 complement resistance protein TraT -
C7A02_RS26420 68629..68964 + 336 Protein_60 colicin -
C7A02_RS26430 69434..69781 + 348 WP_000142426.1 hypothetical protein -
C7A02_RS26435 69799..70389 - 591 WP_000194569.1 hypothetical protein -
C7A02_RS26440 70386..70646 - 261 WP_000343100.1 hypothetical protein -
C7A02_RS26445 71242..71460 + 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C7A02_RS26450 71462..71767 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C7A02_RS26455 71768..72574 + 807 WP_000016966.1 site-specific integrase -
C7A02_RS26460 72754..73398 + 645 WP_001144036.1 ParA family protein -
C7A02_RS26465 73485..73793 + 309 WP_000030199.1 hypothetical protein -
C7A02_RS26470 74208..75188 + 981 WP_000688508.1 plasmid segregation protein ParM -
C7A02_RS26475 75181..75597 + 417 WP_001278809.1 plasmid partitioning/stability family protein -
C7A02_RS26480 75599..75772 - 174 Protein_71 DUF4113 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - espP / hlyC / hlyA / hlyB / hlyD 1..160576 160576


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T99622 WP_001159871.1 NZ_CP027585:71462-71767 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T99622 NZ_CP027585:71462-71767 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT99622 WP_000813639.1 NZ_CP027585:71242-71460 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT99622 NZ_CP027585:71242-71460 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References