Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2911868..2912522 Replicon chromosome
Accession NZ_CP027584
Organism Escherichia coli strain 00-3076

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag C7A02_RS15320 Protein ID WP_000244772.1
Coordinates 2911868..2912275 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C7A02_RS15325 Protein ID WP_000354046.1
Coordinates 2912256..2912522 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7A02_RS15300 2907825..2909558 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
C7A02_RS15305 2909564..2910274 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C7A02_RS15310 2910299..2911195 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C7A02_RS15315 2911307..2911828 + 522 WP_001055874.1 flavodoxin FldB -
C7A02_RS15320 2911868..2912275 - 408 WP_000244772.1 protein YgfX Toxin
C7A02_RS15325 2912256..2912522 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C7A02_RS15330 2912765..2913745 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C7A02_RS15335 2913822..2914481 - 660 WP_000250274.1 hemolysin III family protein -
C7A02_RS15340 2914645..2914956 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
C7A02_RS15345 2915001..2916434 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
C7A02_RS15350 2916491..2917234 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T99614 WP_000244772.1 NZ_CP027584:c2912275-2911868 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T99614 NZ_CP027584:c2912275-2911868 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT99614 WP_000354046.1 NZ_CP027584:c2912522-2912256 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT99614 NZ_CP027584:c2912522-2912256 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References