Detailed information of TA system TA099601

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 915199..915678
• Replicon: chromosome
• Accession: NZ_CP027584
• Organism: Escherichia coli strain 00-3076

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0P7S0Y8
• Locus tag: C7A02_RS04680
• Protein ID: WP_032211256.1
• Coordinates: 915199..915486 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: -
• Locus tag: C7A02_RS04685
• Protein ID: WP_064579147.1
• Coordinates: 915487..915678 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7A02_RS04640	910340..910609	-	270	WP_054250470.1	excisionase	-
C7A02_RS04645	910671..913142	-	2472	WP_096560541.1	exonuclease	-
C7A02_RS04650	913238..913426	-	189	WP_064579146.1	DUF1482 family protein	-
C7A02_RS04655	913423..913611	-	189	WP_000450222.1	cell division inhibitor	-
C7A02_RS04670	914140..914655	+	516	WP_000559918.1	hypothetical protein	-
C7A02_RS04675	914769..914921	-	153	WP_000380318.1	DUF1391 family protein	-
C7A02_RS04680	915199..915486	-	288	WP_032211256.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C7A02_RS04685	915487..915678	-	192	WP_064579147.1	hypothetical protein	Antitoxin
C7A02_RS04690	915647..916108	-	462	WP_064579148.1	helix-turn-helix domain-containing protein	-
C7A02_RS04695	916128..916499	-	372	WP_000448216.1	hypothetical protein	-
C7A02_RS04700	916602..916883	+	282	WP_021577226.1	helix-turn-helix domain-containing protein	-
C7A02_RS04705	916887..917312	+	426	WP_000693827.1	Rha family transcriptional regulator	-
C7A02_RS27060	917381..918412	+	1032	WP_052909572.1	phage replisome organizer	-
C7A02_RS04715	918324..918866	+	543	WP_072130322.1	replication protein	-
C7A02_RS04720	918900..919616	+	717	WP_047082289.1	DUF1627 domain-containing protein	-
C7A02_RS04725	919649..919930	+	282	WP_072094476.1	DNA-binding protein	-
C7A02_RS04730	919927..920232	+	306	WP_053294093.1	DUF4406 domain-containing protein	-
C7A02_RS04735	920380..920562	+	183	WP_022581296.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	stx2A / stx2B	906730..935376	28646

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11013.72 Da
• Isoelectric Point: 6.2118

>T99601 WP_032211256.1 NZ_CP027584:c915486-915199 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWKRLRTCVQPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTISGIE
IVNVVHARRQFPVTP

Nucleotide

• Length: 288 bp

>T99601 NZ_CP027584:c915486-915199 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACTTGGATGATATAACCAGCTATATTGCACGCTTTGACATAGA
TGCGGCAGAACGTTTATGGAAACGCTTAAGGACTTGCGTTCAGCCATTATCTGAGCATCCGTATTTATACCCGCCAAGTG
ACAGAGTGCCAGGTTTGCGTGAAATTGTAGCTCACCCCAATTACATTATTCTGTACCGAGTGACAATTTCAGGCATTGAA
ATTGTTAACGTAGTTCACGCCCGCCGACAGTTCCCTGTAACACCTTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7395.30 Da
• Isoelectric Point: 5.1069

>AT99601 WP_064579147.1 NZ_CP027584:c915678-915487 [Escherichia coli]
MNTTLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDEVERRMAERFAKMRKERSEQ

Nucleotide

• Length: 192 bp

>AT99601 NZ_CP027584:c915678-915487 [Escherichia coli]
ATGAACACAACCCTTTCACCGATGGTTTCTGAATTTGAAACTGTCGAGCAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAGCGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAGGTTGAGCGTAGAATGGCAGAACGCTTTG
CTAAAATGCGCAAGGAACGGAGTGAGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A0P7S0Y8