Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 36156..36799 Replicon plasmid unnamed
Accession NZ_CP027583
Organism Escherichia coli strain 2013C-4538

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag C6999_RS30895 Protein ID WP_001044768.1
Coordinates 36383..36799 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag C6999_RS30890 Protein ID WP_001261287.1
Coordinates 36156..36386 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6999_RS30845 32021..33460 + 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -
C6999_RS30850 33527..33670 + 144 Protein_28 transcriptional regulator -
C6999_RS30855 33674..33865 + 192 Protein_29 lipid II-degrading bacteriocin -
C6999_RS30860 33873..34226 - 354 WP_000864810.1 colicin M immunity protein -
C6999_RS30865 34399..35181 - 783 WP_032277352.1 site-specific integrase -
C6999_RS30870 35183..35596 - 414 WP_000465041.1 hypothetical protein -
C6999_RS30890 36156..36386 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C6999_RS30895 36383..36799 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
C6999_RS30900 36961..37506 - 546 WP_001165114.1 hypothetical protein -
C6999_RS30905 38264..39124 - 861 WP_000704534.1 alpha/beta hydrolase -
C6999_RS30910 39252..39638 + 387 WP_001066949.1 tyrosine-type recombinase/integrase -
C6999_RS30915 39692..40366 + 675 WP_001341423.1 IS66 family insertion sequence hypothetical protein -
C6999_RS30920 40363..40710 + 348 WP_000631725.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / hlyC / hlyA / hlyB / hlyD / toxB 1..88339 88339


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T99598 WP_001044768.1 NZ_CP027583:36383-36799 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T99598 NZ_CP027583:36383-36799 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT99598 WP_001261287.1 NZ_CP027583:36156-36386 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT99598 NZ_CP027583:36156-36386 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References