Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 5291786..5292440 Replicon chromosome
Accession NZ_CP027582
Organism Escherichia coli strain 2013C-4538

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C6999_RS28675 Protein ID WP_000244777.1
Coordinates 5292033..5292440 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6999_RS28670 Protein ID WP_000354046.1
Coordinates 5291786..5292052 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6999_RS28645 5287954..5288265 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C6999_RS28650 5288429..5289088 + 660 WP_000250274.1 hemolysin III family protein -
C6999_RS28655 5289211..5290191 - 981 WP_000399648.1 IS110-like element IS621 family transposase -
C6999_RS28665 5290563..5291543 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
C6999_RS28670 5291786..5292052 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6999_RS28675 5292033..5292440 + 408 WP_000244777.1 toxin CptA Toxin
C6999_RS28680 5292480..5293001 - 522 WP_001055874.1 flavodoxin FldB -
C6999_RS28685 5293113..5294009 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6999_RS28690 5294034..5294744 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6999_RS28695 5294750..5296483 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 5289211..5290191 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T99595 WP_000244777.1 NZ_CP027582:5292033-5292440 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T99595 NZ_CP027582:5292033-5292440 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT99595 WP_000354046.1 NZ_CP027582:5291786-5292052 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT99595 NZ_CP027582:5291786-5292052 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References