Detailed information of TA system TA099590

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3627575..3628407
• Replicon: chromosome
• Accession: NZ_CP027582
• Organism: Escherichia coli strain 2013C-4538

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: C6999_RS19940
• Protein ID: WP_000854753.1
• Coordinates: 3627575..3627949 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: C6999_RS19945
• Protein ID: WP_001315620.1
• Coordinates: 3628039..3628407 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6999_RS19895	3622790..3623896	+	1107	WP_001341302.1	N-acetylneuraminate epimerase	-
C6999_RS19900	3623961..3624941	+	981	WP_000991416.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
C6999_RS19905	3624949..3625599	-	651	WP_001037966.1	HNH endonuclease	-
C6999_RS31740	3626644..3626796	-	153	WP_001280445.1	hypothetical protein	-
C6999_RS19930	3626881..3627078	-	198	WP_000839281.1	DUF957 domain-containing protein	-
C6999_RS19935	3627090..3627578	-	489	WP_000777547.1	hypothetical protein	-
C6999_RS19940	3627575..3627949	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
C6999_RS19945	3628039..3628407	-	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6999_RS19950	3628570..3628791	-	222	WP_000692345.1	DUF987 domain-containing protein	-
C6999_RS19955	3628854..3629330	-	477	WP_001186774.1	RadC family protein	-
C6999_RS19960	3629346..3629831	-	486	WP_000849588.1	antirestriction protein	-
C6999_RS19965	3629886..3630704	-	819	WP_001234622.1	DUF945 domain-containing protein	-
C6999_RS19975	3630804..3631037	-	234	WP_001119729.1	DUF905 family protein	-
C6999_RS19980	3631116..3631571	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T99590 WP_000854753.1 NZ_CP027582:c3627949-3627575 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T99590 NZ_CP027582:c3627949-3627575 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT99590 WP_001315620.1 NZ_CP027582:c3628407-3628039 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT99590 NZ_CP027582:c3628407-3628039 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5