Detailed information of TA system TA099585

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2391261..2392105
• Replicon: chromosome
• Accession: NZ_CP027582
• Organism: Escherichia coli strain 2013C-4538

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: C6999_RS13430
• Protein ID: WP_000854686.1
• Coordinates: 2391261..2391644 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: C6999_RS13435
• Protein ID: WP_001285602.1
• Coordinates: 2391725..2392105 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6999_RS13400	2387937..2388641	+	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-
C6999_RS13405	2388926..2389144	+	219	WP_001040187.1	translation initiation factor IF-1	-
C6999_RS13415	2389635..2390477	-	843	WP_032278432.1	DUF4942 domain-containing protein	-
C6999_RS13420	2390562..2390759	-	198	WP_000839253.1	DUF957 domain-containing protein	-
C6999_RS13425	2390776..2391264	-	489	WP_001054233.1	hypothetical protein	-
C6999_RS13430	2391261..2391644	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
C6999_RS13435	2391725..2392105	-	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6999_RS13440	2392116..2392799	-	684	WP_000086768.1	hypothetical protein	-
C6999_RS13445	2392818..2393039	-	222	WP_000692298.1	DUF987 domain-containing protein	-
C6999_RS13450	2393102..2393578	-	477	WP_001186726.1	RadC family protein	-
C6999_RS13455	2393594..2394079	-	486	WP_000214307.1	antirestriction protein	-
C6999_RS13460	2394171..2394992	-	822	WP_001761104.1	DUF945 domain-containing protein	-
C6999_RS13470	2395093..2395301	-	209	Protein_2471	DUF905 domain-containing protein	-
C6999_RS13475	2395402..2395857	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T99585 WP_000854686.1 NZ_CP027582:c2391644-2391261 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T99585 NZ_CP027582:c2391644-2391261 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT99585 WP_001285602.1 NZ_CP027582:c2392105-2391725 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT99585 NZ_CP027582:c2392105-2391725 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG