Detailed information of TA system TA099583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1912886..1913364
• Replicon: chromosome
• Accession: NZ_CP027582
• Organism: Escherichia coli strain 2013C-4538

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: C6999_RS10480
• Protein ID: WP_001303876.1
• Coordinates: 1913077..1913364 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: C6999_RS10475
• Protein ID: WP_000536233.1
• Coordinates: 1912886..1913077 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6999_RS10440	1908291..1909061	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
C6999_RS10445	1909083..1909829	-	747	WP_000788750.1	ATP-binding protein	-
C6999_RS10450	1909836..1910927	-	1092	WP_001205823.1	hypothetical protein	-
C6999_RS10455	1911006..1911461	+	456	WP_000273724.1	hypothetical protein	-
C6999_RS10460	1911667..1912092	-	426	WP_000693855.1	Rha family transcriptional regulator	-
C6999_RS10465	1912076..1912348	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
C6999_RS10470	1912457..1912858	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
C6999_RS10475	1912886..1913077	+	192	WP_000536233.1	hypothetical protein	Antitoxin
C6999_RS10480	1913077..1913364	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6999_RS10485	1913641..1913796	+	156	WP_000379575.1	DUF1391 family protein	-
C6999_RS10495	1913938..1914327	+	390	WP_000394511.1	hypothetical protein	-
C6999_RS10500	1914514..1914699	-	186	WP_001133046.1	hypothetical protein	-
C6999_RS10510	1915273..1915461	+	189	WP_000413705.1	cell division inhibition protein DicB	-
C6999_RS10515	1915458..1915649	+	192	WP_001098307.1	DUF1482 family protein	-
C6999_RS10520	1915743..1918214	+	2472	WP_032278809.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espM2 / espW	1847682..1944008	96326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T99583 WP_001303876.1 NZ_CP027582:1913077-1913364 [Escherichia coli]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T99583 NZ_CP027582:1913077-1913364 [Escherichia coli]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT99583 WP_000536233.1 NZ_CP027582:1912886-1913077 [Escherichia coli]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT99583 NZ_CP027582:1912886-1913077 [Escherichia coli]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA