Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 979141..979612 Replicon chromosome
Accession NZ_CP027582
Organism Escherichia coli strain 2013C-4538

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag C6999_RS05115 Protein ID WP_001303511.1
Coordinates 979141..979419 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C6999_RS05120 Protein ID WP_001302048.1
Coordinates 979421..979612 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6999_RS05090 974791..977442 - 2652 WP_000105101.1 exodeoxyribonuclease VIII -
C6999_RS05095 977541..977816 - 276 WP_001307773.1 hypothetical protein -
C6999_RS05100 977890..978066 - 177 WP_000245534.1 hypothetical protein -
C6999_RS05105 978060..978281 - 222 WP_000560218.1 killing protein KilR -
C6999_RS05110 978702..978854 - 153 WP_001427316.1 DUF1391 family protein -
C6999_RS05115 979141..979419 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6999_RS05120 979421..979612 - 192 WP_001302048.1 hypothetical protein Antitoxin
C6999_RS05125 979633..980004 - 372 WP_001169687.1 hypothetical protein -
C6999_RS05130 980103..980405 + 303 WP_000172738.1 transcriptional regulator -
C6999_RS05135 980402..980827 + 426 WP_000693943.1 Rha family transcriptional regulator -
C6999_RS05140 980850..981812 + 963 WP_001444941.1 helix-turn-helix domain-containing protein -
C6999_RS05145 981819..982560 + 742 Protein_957 ATP-binding protein -
C6999_RS05150 982586..983347 + 762 WP_000450872.1 DUF1627 domain-containing protein -
C6999_RS05155 983380..983661 + 282 WP_000603384.1 DNA-binding protein -
C6999_RS05160 983658..983885 + 228 WP_000699809.1 hypothetical protein -
C6999_RS05165 983878..984189 + 312 WP_001289673.1 hypothetical protein -
C6999_RS05170 984317..984535 + 219 WP_000683609.1 DUF4014 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 964352..1034677 70325


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T99580 WP_001303511.1 NZ_CP027582:c979419-979141 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T99580 NZ_CP027582:c979419-979141 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT99580 WP_001302048.1 NZ_CP027582:c979612-979421 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT99580 NZ_CP027582:c979612-979421 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References