Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 725893..726724 Replicon chromosome
Accession NZ_CP027582
Organism Escherichia coli strain 2013C-4538

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag C6999_RS03760 Protein ID WP_000854814.1
Coordinates 725893..726267 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag C6999_RS03765 Protein ID WP_001285585.1
Coordinates 726356..726724 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6999_RS03720 721289..722455 + 1167 WP_000830150.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
C6999_RS03725 722574..723047 + 474 WP_001105389.1 DNA gyrase inhibitor SbmC -
C6999_RS03730 723245..724303 + 1059 WP_001200891.1 FUSC family protein -
C6999_RS03735 724475..724804 + 330 WP_000450409.1 DUF496 family protein -
C6999_RS03740 724905..725087 - 183 WP_001016348.1 hypothetical protein -
C6999_RS03745 725159..725287 + 129 Protein_701 transposase domain-containing protein -
C6999_RS31640 725576..725689 - 114 WP_001161660.1 DUF957 domain-containing protein -
C6999_RS03755 725702..725896 - 195 WP_000988600.1 hypothetical protein -
C6999_RS03760 725893..726267 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
C6999_RS03765 726356..726724 - 369 WP_001285585.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
C6999_RS03770 726798..727019 - 222 WP_000692323.1 DUF987 domain-containing protein -
C6999_RS03775 727082..727558 - 477 WP_001186726.1 RadC family protein -
C6999_RS03780 727574..728053 - 480 WP_000860087.1 antirestriction protein -
C6999_RS03785 728135..728953 - 819 WP_001234629.1 DUF945 domain-containing protein -
C6999_RS03795 729053..729286 - 234 WP_001119729.1 DUF905 family protein -
C6999_RS03800 729365..729820 - 456 WP_000581504.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T99567 WP_000854814.1 NZ_CP027582:c726267-725893 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T99567 NZ_CP027582:c726267-725893 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT99567 WP_001285585.1 NZ_CP027582:c726724-726356 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT99567 NZ_CP027582:c726724-726356 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References