Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 57308..57833 Replicon plasmid unnamed2
Accession NZ_CP027581
Organism Escherichia coli strain 2013C-4282

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C6996_RS26075 Protein ID WP_001159871.1
Coordinates 57528..57833 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag C6996_RS26070 Protein ID WP_000813639.1
Coordinates 57308..57526 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6996_RS26030 53485..53595 + 111 Protein_49 IS481 family transposase -
C6996_RS26040 53867..54598 + 732 WP_000850414.1 complement resistance protein TraT -
C6996_RS26045 54658..55029 + 372 WP_052892432.1 colicin -
C6996_RS26055 55512..55859 + 348 WP_000142427.1 hypothetical protein -
C6996_RS26060 55877..56467 - 591 WP_052892431.1 hypothetical protein -
C6996_RS26065 56464..56712 - 249 WP_052892430.1 hypothetical protein -
C6996_RS26070 57308..57526 + 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C6996_RS26075 57528..57833 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C6996_RS26080 57834..58643 + 810 WP_052892429.1 site-specific integrase -
C6996_RS26085 58781..59056 - 276 WP_000239527.1 hypothetical protein -
C6996_RS26090 59050..59694 - 645 WP_000633912.1 AAA family ATPase -
C6996_RS26095 59923..60894 + 972 WP_001103695.1 hypothetical protein -
C6996_RS26100 60899..61291 + 393 WP_000340836.1 plasmid partitioning/stability family protein -
C6996_RS26110 61296..62566 - 1271 Protein_62 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - espP / hlyC / hlyA / hlyB / hlyD 1..118822 118822


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T99560 WP_001159871.1 NZ_CP027581:57528-57833 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T99560 NZ_CP027581:57528-57833 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCTGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT99560 WP_000813639.1 NZ_CP027581:57308-57526 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT99560 NZ_CP027581:57308-57526 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References