Detailed information of TA system TA099559

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 38358..38905
• Replicon: plasmid unnamed1
• Accession: NZ_CP027580
• Organism: Escherichia coli strain 2013C-4282

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: C6996_RS25565
• Protein ID: WP_000970004.1
• Coordinates: 38624..38905 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q5JBQ7
• Locus tag: C6996_RS25560
• Protein ID: WP_000079943.1
• Coordinates: 38358..38627 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6996_RS25510	34366..34827	+	462	WP_000760082.1	thermonuclease family protein	-
-	35486..35542	-	57	NuclAT_1	-	-
-	35486..35542	-	57	NuclAT_1	-	-
-	35486..35542	-	57	NuclAT_1	-	-
-	35486..35542	-	57	NuclAT_1	-	-
C6996_RS25525	35534..35740	+	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	-
C6996_RS26785	36024..36272	+	249	Protein_39	replication regulatory protein RepA	-
C6996_RS25540	36519..36593	+	75	WP_001365571.1	RepA leader peptide Tap	-
C6996_RS25545	36586..37443	+	858	WP_000130991.1	incFII family plasmid replication initiator RepA	-
C6996_RS25560	38358..38627	+	270	WP_000079943.1	hypothetical protein	Antitoxin
C6996_RS25565	38624..38905	+	282	WP_000970004.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6996_RS25570	39014..39364	-	351	WP_001365718.1	plasmid partitioning/stability family protein	-
C6996_RS25575	39367..40329	-	963	WP_000959884.1	plasmid segregation protein ParM	-
C6996_RS25585	40879..41781	+	903	WP_088137943.1	DUF1281 domain-containing protein	-
C6996_RS25590	41778..42090	+	313	Protein_47	hypothetical protein	-
C6996_RS25595	42166..42849	+	684	WP_000086155.1	DNA methylase	-
C6996_RS25600	42850..43071	+	222	WP_001104895.1	hypothetical protein	-
C6996_RS25605	43085..43519	+	435	WP_000274463.1	DUF1380 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..54544	54544

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10846.54 Da
• Isoelectric Point: 8.4615

>T99559 WP_000970004.1 NZ_CP027580:38624-38905 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLVAAIELDERIGYSASSLAGLPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T99559 NZ_CP027580:38624-38905 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGGT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCTGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10272.57 Da
• Isoelectric Point: 7.2057

>AT99559 WP_000079943.1 NZ_CP027580:38358-38627 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLSHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT99559 NZ_CP027580:38358-38627 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGTCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGCCAGCAACTTGAGCGCGGTGATGTGCTTTCCCATGACATGGTCGAATCCTCAGCTACCGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A622US83