Detailed information of TA system TA099558

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4795197..4795979
• Replicon: chromosome
• Accession: NZ_CP027579
• Organism: Escherichia coli strain 2013C-4282

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: C6996_RS24170
• Protein ID: WP_042944403.1
• Coordinates: 4795197..4795571 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C6996_RS24175
• Protein ID: WP_042944401.1
• Coordinates: 4795623..4795979 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6996_RS24150	4793085..4793933	-	849	WP_102000580.1	DUF4942 domain-containing protein	-
C6996_RS24155	4794012..4794206	-	195	WP_102000579.1	DUF957 domain-containing protein	-
C6996_RS24160	4794236..4794727	-	492	WP_106907770.1	hypothetical protein	-
C6996_RS24165	4794724..4795101	-	378	WP_042944406.1	TA system toxin CbtA family protein	-
C6996_RS24170	4795197..4795571	-	375	WP_042944403.1	TA system toxin CbtA family protein	Toxin
C6996_RS24175	4795623..4795979	-	357	WP_042944401.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6996_RS24180	4796031..4796675	-	645	WP_106907771.1	antitoxin of toxin-antitoxin stability system	-
C6996_RS24185	4796691..4796912	-	222	WP_042944397.1	DUF987 domain-containing protein	-
C6996_RS24190	4796927..4797406	-	480	WP_102000576.1	DNA repair protein RadC	-
C6996_RS24195	4797418..4797888	-	471	WP_023567995.1	antirestriction protein	-
C6996_RS24200	4797969..4798793	-	825	WP_201309429.1	DUF945 domain-containing protein	-
C6996_RS24205	4798901..4799134	-	234	WP_042944390.1	DUF905 domain-containing protein	-
C6996_RS24210	4799222..4800166	-	945	WP_102000585.1	hypothetical protein	-
C6996_RS24215	4800219..4800434	-	216	WP_042944388.1	hypothetical protein	-
C6996_RS26765	4800446..4800595	-	150	WP_001458941.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (18-110)

Antitoxin

• Antitoxin CbeA (15-113)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13571.26 Da
• Isoelectric Point: 4.5407

>T99558 WP_042944403.1 NZ_CP027579:c4795571-4795197 [Escherichia coli]
MRIYPVSAKRAAEIERLSPVSPVAVWQRLLTYLLDRHYGLTLNDTQFGDDGTIQECIDVGDPLLDALNEVVEDDELERTD
NRGKSLLFRSSLLTPADIRNAQKAVGLAGNSASLIISGQSSARS

Nucleotide

• Length: 375 bp

>T99558 NZ_CP027579:c4795571-4795197 [Escherichia coli]
ATGAGAATTTATCCTGTATCCGCAAAACGGGCCGCTGAGATTGAGCGGTTATCGCCTGTGTCACCGGTTGCCGTCTGGCA
GCGCCTCCTGACGTACCTGCTGGACCGTCACTACGGTCTGACGCTTAACGATACTCAGTTTGGCGATGACGGCACCATTC
AGGAATGCATTGACGTCGGTGATCCCCTGCTTGACGCCCTGAACGAAGTGGTTGAAGACGACGAGCTGGAGCGTACTGAT
AACCGGGGAAAATCACTTCTGTTCAGATCGTCGCTGCTCACACCCGCAGATATCCGGAACGCCCAGAAAGCCGTGGGTCT
GGCAGGCAACAGCGCCTCCCTCATTATCAGCGGTCAGTCGTCAGCCAGAAGCTAA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13079.82 Da
• Isoelectric Point: 6.3170

>AT99558 WP_042944401.1 NZ_CP027579:c4795979-4795623 [Escherichia coli]
MPDIPATNDNMDAPRWGLQRNITPCFGARLVQEGNRLHYLADRASINGTFSDADLRHLDQSFPLLLKQLELMLVSGELNP
RHQHSVTLYAKGLTCEADSLASHGYVYIAIYPTPATTA

Nucleotide

• Length: 357 bp

>AT99558 NZ_CP027579:c4795979-4795623 [Escherichia coli]
ATGCCAGATATCCCTGCCACAAATGACAATATGGACGCGCCGCGCTGGGGTCTTCAGCGCAATATCACGCCCTGTTTTGG
CGCTCGCCTGGTGCAGGAGGGCAACCGGCTGCATTACCTGGCTGACCGCGCCAGTATCAACGGAACCTTCAGCGATGCGG
ATTTACGCCATCTCGACCAGTCGTTTCCGCTGCTGTTGAAACAACTGGAACTGATGCTGGTTTCCGGTGAACTCAATCCC
CGTCATCAACACAGCGTTACGCTTTACGCAAAAGGGCTGACCTGCGAGGCCGACAGCCTCGCTTCGCACGGGTACGTCTA
TATCGCAATTTATCCGACACCAGCCACAACCGCATAA