Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4496584..4497238 Replicon chromosome
Accession NZ_CP027579
Organism Escherichia coli strain 2013C-4282

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag C6996_RS22725 Protein ID WP_000244781.1
Coordinates 4496831..4497238 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6996_RS22720 Protein ID WP_000354046.1
Coordinates 4496584..4496850 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6996_RS22695 4491744..4492487 + 744 WP_000951966.1 SDR family oxidoreductase -
C6996_RS22700 4492544..4493977 - 1434 WP_001605840.1 6-phospho-beta-glucosidase BglA -
C6996_RS22705 4494022..4494333 + 312 WP_001182943.1 N(4)-acetylcytidine aminohydrolase -
C6996_RS22710 4494497..4495156 + 660 WP_000250283.1 hemolysin III family protein -
C6996_RS22715 4495352..4496332 - 981 WP_000886102.1 tRNA-modifying protein YgfZ -
C6996_RS22720 4496584..4496850 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6996_RS22725 4496831..4497238 + 408 WP_000244781.1 protein YgfX Toxin
C6996_RS22730 4497278..4497799 - 522 WP_001055874.1 flavodoxin FldB -
C6996_RS22735 4497911..4498807 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6996_RS22740 4498832..4499542 + 711 WP_000715223.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6996_RS22745 4499548..4501281 + 1734 WP_000813232.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T99556 WP_000244781.1 NZ_CP027579:4496831-4497238 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T99556 NZ_CP027579:4496831-4497238 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT99556 WP_000354046.1 NZ_CP027579:4496584-4496850 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT99556 NZ_CP027579:4496584-4496850 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGAATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAGCTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References