Detailed information of TA system TA099552

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 2917706..2918413
• Replicon: chromosome
• Accession: NZ_CP027579
• Organism: Escherichia coli strain 2013C-4282

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: C6996_RS14805
• Protein ID: WP_106907648.1
• Coordinates: 2917706..2918047 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: C6996_RS14810
• Protein ID: WP_000939437.1
• Coordinates: 2918078..2918413 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6996_RS14785	2915243..2916040	-	798	WP_106907645.1	helix-turn-helix transcriptional regulator	-
C6996_RS14790	2916155..2916430	-	276	WP_106907646.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C6996_RS14795	2916434..2916682	-	249	WP_000535212.1	ribbon-helix-helix domain-containing protein	-
C6996_RS14800	2916758..2917591	-	834	WP_106907647.1	DUF4942 domain-containing protein	-
C6996_RS14805	2917706..2918047	-	342	WP_106907648.1	TA system toxin CbtA family protein	Toxin
C6996_RS14810	2918078..2918413	-	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6996_RS14815	2918413..2918886	-	474	WP_001292742.1	DNA repair protein RadC	-
C6996_RS14820	2918916..2919734	-	819	WP_106907649.1	DUF945 domain-containing protein	-
C6996_RS14825	2919970..2920923	-	954	WP_000290407.1	hypothetical protein	-
C6996_RS14830	2921516..2922130	+	615	WP_106907650.1	inovirus Gp2 family protein	-
C6996_RS14835	2922248..2922466	+	219	WP_001064742.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimC / fimI / fimA / fimE / fimB	2902290..2932689	30399

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13076.13 Da
• Isoelectric Point: 9.9065

>T99552 WP_106907648.1 NZ_CP027579:c2918047-2917706 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAINFLVEKYELIRIDRKGF
SWREQTPYLRAVDILRARQATGLLRRCHNTTAR

Nucleotide

• Length: 342 bp

>T99552 NZ_CP027579:c2918047-2917706 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCCGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGACTGAACCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTTGATGCCATCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCGGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCTACAGGTCTGCTGCGTCGCTG
CCATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT99552 WP_000939437.1 NZ_CP027579:c2918413-2918078 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT99552 NZ_CP027579:c2918413-2918078 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATTACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGTTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6