Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2234203..2234821 Replicon chromosome
Accession NZ_CP027579
Organism Escherichia coli strain 2013C-4282

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C6996_RS11415 Protein ID WP_001291435.1
Coordinates 2234603..2234821 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C6996_RS11410 Protein ID WP_000344800.1
Coordinates 2234203..2234577 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6996_RS11400 2229291..2230484 + 1194 WP_001353399.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C6996_RS11405 2230507..2233656 + 3150 WP_001132492.1 multidrug efflux RND transporter permease subunit -
C6996_RS11410 2234203..2234577 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C6996_RS11415 2234603..2234821 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C6996_RS11420 2234993..2235544 + 552 WP_000102551.1 maltose O-acetyltransferase -
C6996_RS11425 2235660..2236130 + 471 WP_000136192.1 YlaC family protein -
C6996_RS11430 2236294..2237844 + 1551 WP_001353400.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C6996_RS11435 2237886..2238239 - 354 WP_000878140.1 DUF1428 domain-containing protein -
C6996_RS11445 2238618..2238929 + 312 WP_000409907.1 MGMT family protein -
C6996_RS11450 2238960..2239532 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T99548 WP_001291435.1 NZ_CP027579:2234603-2234821 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T99548 NZ_CP027579:2234603-2234821 [Escherichia coli]
ATGTCCGAAAAACCTTTAACCAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT99548 WP_000344800.1 NZ_CP027579:2234203-2234577 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT99548 NZ_CP027579:2234203-2234577 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGATTGCCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAAAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References