99535

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 5307307..5307961 Replicon chromosome
Accession NZ_CP027577
Organism Escherichia coli strain 2013C-4225

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C6997_RS28760 Protein ID WP_000244777.1
Coordinates 5307307..5307714 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6997_RS28765 Protein ID WP_000354046.1
Coordinates 5307695..5307961 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6997_RS28740 5303264..5304997 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
C6997_RS28745 5305003..5305713 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6997_RS28750 5305738..5306634 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6997_RS28755 5306746..5307267 + 522 WP_001055874.1 flavodoxin FldB -
C6997_RS28760 5307307..5307714 - 408 WP_000244777.1 toxin CptA Toxin
C6997_RS28765 5307695..5307961 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6997_RS28770 5308204..5309184 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
C6997_RS28775 5309380..5310039 - 660 WP_000250274.1 hemolysin III family protein -
C6997_RS28780 5310203..5310514 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C6997_RS28785 5310559..5311992 + 1434 WP_001341859.1 6-phospho-beta-glucosidase BglA -
C6997_RS28790 5312049..5312792 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T99535 WP_000244777.1 NZ_CP027577:c5307714-5307307 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T99535 NZ_CP027577:c5307714-5307307 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT99535 WP_000354046.1 NZ_CP027577:c5307961-5307695 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT99535 NZ_CP027577:c5307961-5307695 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QD57

References