Detailed information of TA system TA099522

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3502162..3502800
• Replicon: chromosome
• Accession: NZ_CP027577
• Organism: Escherichia coli strain 2013C-4225

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: C6997_RS19055
• Protein ID: WP_000813794.1
• Coordinates: 3502624..3502800 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C6997_RS19050
• Protein ID: WP_001270286.1
• Coordinates: 3502162..3502578 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6997_RS19030	3497315..3498256	-	942	WP_001251332.1	ABC transporter permease	-
C6997_RS19035	3498257..3499270	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
C6997_RS19040	3499288..3500433	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
C6997_RS19045	3500678..3502083	-	1406	Protein_3435	PLP-dependent aminotransferase family protein	-
C6997_RS19050	3502162..3502578	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C6997_RS19055	3502624..3502800	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C6997_RS19060	3503022..3503252	+	231	WP_000494244.1	YncJ family protein	-
C6997_RS19065	3503344..3505305	-	1962	WP_001341357.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C6997_RS19070	3505378..3505914	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
C6997_RS19075	3505967..3507181	+	1215	WP_001307190.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3507221..3508369	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T99522 WP_000813794.1 NZ_CP027577:c3502800-3502624 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T99522 NZ_CP027577:c3502800-3502624 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT99522 WP_001270286.1 NZ_CP027577:c3502578-3502162 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT99522 NZ_CP027577:c3502578-3502162 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB