Detailed information of TA system TA099514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2975080..2975558
• Replicon: chromosome
• Accession: NZ_CP027577
• Organism: Escherichia coli strain 2013C-4225

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: C6997_RS15970
• Protein ID: WP_001303876.1
• Coordinates: 2975080..2975367 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: C6997_RS15975
• Protein ID: WP_000536233.1
• Coordinates: 2975367..2975558 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6997_RS15930	2970230..2972701	-	2472	WP_032317593.1	exonuclease	-
C6997_RS15935	2972795..2972986	-	192	WP_001098307.1	DUF1482 family protein	-
C6997_RS15940	2972983..2973171	-	189	WP_000413705.1	cell division inhibition protein DicB	-
C6997_RS15950	2973745..2973930	+	186	WP_001133046.1	hypothetical protein	-
C6997_RS15955	2974117..2974506	-	390	WP_000394511.1	hypothetical protein	-
C6997_RS15965	2974648..2974803	-	156	WP_000379575.1	DUF1391 family protein	-
C6997_RS15970	2975080..2975367	-	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6997_RS15975	2975367..2975558	-	192	WP_000536233.1	hypothetical protein	Antitoxin
C6997_RS15980	2975586..2975987	-	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
C6997_RS15985	2976096..2976368	+	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
C6997_RS15990	2976352..2976777	+	426	WP_000693855.1	Rha family transcriptional regulator	-
C6997_RS15995	2976983..2977438	-	456	WP_000273724.1	hypothetical protein	-
C6997_RS16000	2977517..2978608	+	1092	WP_077756695.1	DNA-binding protein	-
C6997_RS16005	2978615..2979361	+	747	WP_000788750.1	ATP-binding protein	-
C6997_RS16010	2979383..2980153	+	771	WP_000450992.1	DUF1627 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espW / espM2	2947403..3043545	96142

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T99514 WP_001303876.1 NZ_CP027577:c2975367-2975080 [Escherichia coli]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T99514 NZ_CP027577:c2975367-2975080 [Escherichia coli]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT99514 WP_000536233.1 NZ_CP027577:c2975558-2975367 [Escherichia coli]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT99514 NZ_CP027577:c2975558-2975367 [Escherichia coli]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA