99512

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2017908..2018526 Replicon chromosome
Accession NZ_CP027577
Organism Escherichia coli strain 2013C-4225

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C6997_RS10565 Protein ID WP_001291435.1
Coordinates 2017908..2018126 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C6997_RS10570 Protein ID WP_000344800.1
Coordinates 2018152..2018526 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6997_RS10530 2013198..2013770 + 573 WP_023442208.1 YbaY family lipoprotein -
C6997_RS10535 2013801..2014112 - 312 WP_000409911.1 MGMT family protein -
C6997_RS10545 2014490..2014843 + 354 WP_023442207.1 DUF1428 family protein -
C6997_RS10550 2014885..2016435 - 1551 WP_001342068.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C6997_RS10555 2016599..2017069 - 471 WP_000136192.1 YlaC family protein -
C6997_RS10560 2017185..2017736 - 552 WP_000102564.1 maltose O-acetyltransferase -
C6997_RS10565 2017908..2018126 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C6997_RS10570 2018152..2018526 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C6997_RS10575 2019072..2022221 - 3150 WP_001132475.1 multidrug efflux RND transporter permease subunit -
C6997_RS10580 2022244..2023437 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T99512 WP_001291435.1 NZ_CP027577:c2018126-2017908 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T99512 NZ_CP027577:c2018126-2017908 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT99512 WP_000344800.1 NZ_CP027577:c2018526-2018152 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT99512 NZ_CP027577:c2018526-2018152 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References