Detailed information of TA system TA099507

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1376647..1377479
• Replicon: chromosome
• Accession: NZ_CP027577
• Organism: Escherichia coli strain 2013C-4225

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: C6997_RS07320
• Protein ID: WP_000854753.1
• Coordinates: 1377105..1377479 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: C6997_RS07315
• Protein ID: WP_001315620.1
• Coordinates: 1376647..1377015 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6997_RS07280	1373483..1373938	+	456	WP_000581504.1	hypothetical protein	-
C6997_RS07285	1374017..1374250	+	234	WP_001119729.1	DUF905 family protein	-
C6997_RS07295	1374350..1375168	+	819	WP_001234622.1	DUF945 domain-containing protein	-
C6997_RS07300	1375223..1375708	+	486	WP_000849588.1	antirestriction protein	-
C6997_RS07305	1375724..1376200	+	477	WP_001186774.1	RadC family protein	-
C6997_RS07310	1376263..1376484	+	222	WP_000692345.1	DUF987 domain-containing protein	-
C6997_RS07315	1376647..1377015	+	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6997_RS07320	1377105..1377479	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
C6997_RS07325	1377476..1377964	+	489	WP_000777547.1	hypothetical protein	-
C6997_RS07330	1377976..1378173	+	198	WP_000839281.1	DUF957 domain-containing protein	-
C6997_RS31530	1378258..1378410	+	153	WP_001280445.1	hypothetical protein	-
C6997_RS07355	1379455..1380105	+	651	WP_001037966.1	HNH endonuclease	-
C6997_RS07360	1380113..1381093	-	981	WP_106918322.1	sialate O-acetylesterase	-
C6997_RS07365	1381158..1382264	-	1107	WP_001341302.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T99507 WP_000854753.1 NZ_CP027577:1377105-1377479 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T99507 NZ_CP027577:1377105-1377479 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT99507 WP_001315620.1 NZ_CP027577:1376647-1377015 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT99507 NZ_CP027577:1376647-1377015 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5