99505

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1116362..1116859 Replicon chromosome
Accession NZ_CP027577
Organism Escherichia coli strain 2013C-4225

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag C6997_RS05890 Protein ID WP_001173343.1
Coordinates 1116686..1116859 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag C6997_RS05885 Protein ID WP_000398619.1
Coordinates 1116362..1116658 (+) Length 99 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6997_RS05860 1113093..1113812 - 720 WP_000611288.1 two-component system response regulator DcuR -
C6997_RS05865 1113809..1115440 - 1632 WP_001216487.1 sensor histidine kinase -
C6997_RS05870 1115621..1115851 + 231 WP_000371700.1 (4Fe-4S)-binding protein -
C6997_RS05875 1115863..1116135 + 273 WP_000405647.1 N-acetyltransferase -
C6997_RS05885 1116362..1116658 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
C6997_RS05890 1116686..1116859 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
C6997_RS05895 1116978..1118495 - 1518 WP_001295074.1 lysine--tRNA ligase -
C6997_RS05900 1118732..1120189 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-57)

Antitoxin

(5-94)


Sequences


Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T99505 WP_001173343.1 NZ_CP027577:1116686-1116859 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T99505 NZ_CP027577:1116686-1116859 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT99505 WP_000398619.1 NZ_CP027577:1116362-1116658 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT99505 NZ_CP027577:1116362-1116658 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBH6

References