Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 93276..93877 Replicon plasmid unnamed2
Accession NZ_CP027575
Organism Escherichia coli strain 2013C-4081

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag A2F94_RS29945 Protein ID WP_001216034.1
Coordinates 93276..93656 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag A2F94_RS29950 Protein ID WP_001190712.1
Coordinates 93656..93877 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A2F94_RS31355 88382..88612 - 231 Protein_94 ash family protein -
A2F94_RS29920 88717..90201 - 1485 WP_000124153.1 hypothetical protein -
A2F94_RS29925 90201..91394 - 1194 WP_000219603.1 terminase -
A2F94_RS29930 91480..91932 - 453 WP_001448129.1 hypothetical protein -
A2F94_RS29935 92021..93064 - 1044 WP_000648823.1 DUF968 domain-containing protein -
A2F94_RS29940 93092..93271 - 180 WP_000113018.1 hypothetical protein -
A2F94_RS29945 93276..93656 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
A2F94_RS29950 93656..93877 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
A2F94_RS29955 93950..94339 - 390 WP_000506726.1 DNA repair protein -
A2F94_RS29960 94514..94849 + 336 WP_001133672.1 hypothetical protein -
A2F94_RS29965 94880..95095 + 216 WP_001048304.1 hypothetical protein -
A2F94_RS29975 95610..95914 - 305 Protein_105 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..95952 95952


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T99498 WP_001216034.1 NZ_CP027575:c93656-93276 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T99498 NZ_CP027575:c93656-93276 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT99498 WP_001190712.1 NZ_CP027575:c93877-93656 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT99498 NZ_CP027575:c93877-93656 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References