Detailed information of TA system TA099497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 30746..31268
• Replicon: plasmid unnamed1
• Accession: NZ_CP027574
• Organism: Escherichia coli strain 2013C-4081

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: A2F94_RS29250
• Protein ID: WP_000220563.1
• Coordinates: 30987..31268 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: A2F94_RS29245
• Protein ID: WP_000121743.1
• Coordinates: 30746..30997 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2F94_RS29215	25824..26486	+	663	WP_000516402.1	peptidyl-arginine deiminase	-
A2F94_RS29220	26584..26865	+	282	WP_001185482.1	hypothetical protein	-
A2F94_RS29225	26890..27867	+	978	WP_001269910.1	hypothetical protein	-
A2F94_RS29230	27864..28289	-	426	WP_000202944.1	hypothetical protein	-
A2F94_RS29235	28357..28803	-	447	WP_001074385.1	hypothetical protein	-
A2F94_RS29240	28828..29682	-	855	WP_032328461.1	replication initiation protein	-
A2F94_RS29245	30746..30997	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
A2F94_RS29250	30987..31268	+	282	WP_000220563.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A2F94_RS29255	31414..31737	+	324	WP_001181904.1	hypothetical protein	-
A2F94_RS29260	31782..32027	+	246	WP_000356542.1	hypothetical protein	-
A2F94_RS29265	32017..32232	+	216	WP_001180117.1	hypothetical protein	-
A2F94_RS29270	32325..32654	+	330	WP_024222272.1	hypothetical protein	-
A2F94_RS29275	32697..32876	+	180	WP_000439079.1	hypothetical protein	-
A2F94_RS29280	33109..33381	+	273	WP_000167280.1	hypothetical protein	-
A2F94_RS31145	33632..33706	+	75	Protein_42	DNA distortion polypeptide 3	-
A2F94_RS31345	33701..34247	+	547	Protein_43	DNA distortion polypeptide 1	-
A2F94_RS29295	34250..35416	+	1167	WP_024222271.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48183	48183

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11021.83 Da
• Isoelectric Point: 10.5938

>T99497 WP_000220563.1 NZ_CP027574:30987-31268 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHSDRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T99497 NZ_CP027574:30987-31268 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATTCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGTTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT99497 WP_000121743.1 NZ_CP027574:30746-30997 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT99497 NZ_CP027574:30746-30997 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A3S5I301