Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2679670..2680167 Replicon chromosome
Accession NZ_CP027573
Organism Escherichia coli strain 2013C-4081

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag A2F94_RS14375 Protein ID WP_001173343.1
Coordinates 2679670..2679843 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag A2F94_RS14380 Protein ID WP_000398619.1
Coordinates 2679871..2680167 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A2F94_RS14360 2677367..2677528 + 162 Protein_2632 IS256 family transposase -
A2F94_RS14370 2678811..2679551 + 741 Protein_2634 lysine--tRNA ligase -
A2F94_RS14375 2679670..2679843 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
A2F94_RS14380 2679871..2680167 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
A2F94_RS14390 2680394..2680666 - 273 WP_000405647.1 N-acetyltransferase -
A2F94_RS14395 2680678..2680908 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
A2F94_RS14400 2681089..2682720 + 1632 WP_001216487.1 sensor histidine kinase -
A2F94_RS14405 2682717..2683436 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - espL2 / espL2 / espL2 / nleB1 / nleE / efa1 / efa1 2659298..2680167 20869


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T99489 WP_001173343.1 NZ_CP027573:c2679843-2679670 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T99489 NZ_CP027573:c2679843-2679670 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT99489 WP_000398619.1 NZ_CP027573:c2680167-2679871 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT99489 NZ_CP027573:c2680167-2679871 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References