99485

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1899188..1900025 Replicon chromosome
Accession NZ_CP027573
Organism Escherichia coli strain 2013C-4081

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag A2F94_RS10440 Protein ID WP_000227784.1
Coordinates 1899483..1900025 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag A2F94_RS10435 Protein ID WP_001297137.1
Coordinates 1899188..1899499 (+) Length 104 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A2F94_RS10410 1894208..1895155 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
A2F94_RS10415 1895177..1897168 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
A2F94_RS10420 1897158..1897772 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
A2F94_RS10425 1897772..1898101 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
A2F94_RS10430 1898113..1899003 + 891 WP_000971336.1 heme o synthase -
A2F94_RS10435 1899188..1899499 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
A2F94_RS10440 1899483..1900025 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
A2F94_RS10445 1900081..1901016 - 936 WP_001345016.1 sel1 repeat family protein -
A2F94_RS10450 1901424..1902788 + 1365 WP_001000988.1 MFS transporter -
A2F94_RS10455 1902916..1903407 - 492 WP_001138904.1 nucleotide binding protein YajQ -
A2F94_RS10460 1903575..1904486 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(68-156)

Antitoxin

(14-97)


Sequences


Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T99485 WP_000227784.1 NZ_CP027573:1899483-1900025 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T99485 NZ_CP027573:1899483-1900025 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT99485 WP_001297137.1 NZ_CP027573:1899188-1899499 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT99485 NZ_CP027573:1899188-1899499 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 7BYY


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References