Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1865110..1865728 Replicon chromosome
Accession NZ_CP027573
Organism Escherichia coli strain 2013C-4081

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag A2F94_RS10265 Protein ID WP_001291435.1
Coordinates 1865510..1865728 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag A2F94_RS10260 Protein ID WP_000344800.1
Coordinates 1865110..1865484 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A2F94_RS10250 1860199..1861392 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
A2F94_RS10255 1861415..1864564 + 3150 WP_001132475.1 multidrug efflux RND transporter permease subunit -
A2F94_RS10260 1865110..1865484 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
A2F94_RS10265 1865510..1865728 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
A2F94_RS10270 1865900..1866451 + 552 WP_000102564.1 maltose O-acetyltransferase -
A2F94_RS10275 1866567..1867037 + 471 WP_001345013.1 YlaC family protein -
A2F94_RS10280 1867201..1868751 + 1551 WP_001369170.1 cyclic-guanylate-specific phosphodiesterase PdeB -
A2F94_RS10285 1868793..1869146 - 354 WP_000878140.1 DUF1428 domain-containing protein -
A2F94_RS10295 1869525..1869836 + 312 WP_000409911.1 MGMT family protein -
A2F94_RS10300 1869867..1870439 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T99484 WP_001291435.1 NZ_CP027573:1865510-1865728 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T99484 NZ_CP027573:1865510-1865728 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT99484 WP_000344800.1 NZ_CP027573:1865110-1865484 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT99484 NZ_CP027573:1865110-1865484 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References