Detailed information of TA system TA099468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5557180..5557705
• Replicon: chromosome
• Accession: NZ_CP027561
• Organism: Pseudomonas fluorescens strain G7

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C6Y56_RS25320
• Protein ID: WP_169432089.1
• Coordinates: 5557409..5557705 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C6Y56_RS25315
• Protein ID: WP_085732973.1
• Coordinates: 5557180..5557419 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6Y56_RS25290	5552955..5553416	+	462	WP_169432085.1	GNAT family N-acetyltransferase	-
C6Y56_RS25295	5553654..5554304	+	651	WP_169432086.1	DedA family protein	-
C6Y56_RS25300	5554309..5555121	+	813	WP_169432087.1	zinc-dependent peptidase	-
C6Y56_RS25305	5555234..5555761	+	528	WP_007933577.1	inorganic diphosphatase	-
C6Y56_RS25310	5555983..5556723	+	741	WP_169432088.1	helix-turn-helix transcriptional regulator	-
C6Y56_RS25315	5557180..5557419	+	240	WP_085732973.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
C6Y56_RS25320	5557409..5557705	+	297	WP_169432089.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6Y56_RS25325	5557765..5558577	-	813	WP_169432090.1	DUF2135 domain-containing protein	-
C6Y56_RS25330	5558581..5560200	-	1620	WP_169432091.1	DUF2300 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-81)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-62)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11599.39 Da
• Isoelectric Point: 9.6729

>T99468 WP_169432089.1 NZ_CP027561:5557409-5557705 [Pseudomonas fluorescens]
MTYDLEFDRRALKEWNKLGDTIREQFKKKLAEVLKNPRIEANRLRELPDCYKVKLKSAGYRLIYQVIDHEIVVFVVAIGK
REREAAYDTAQDRLSSQP

Nucleotide

• Length: 297 bp

>T99468 NZ_CP027561:5557409-5557705 [Pseudomonas fluorescens]
ATGACCTATGACCTCGAGTTTGATCGTCGTGCATTGAAGGAATGGAACAAGCTGGGCGATACGATTCGCGAGCAGTTCAA
AAAGAAACTCGCCGAGGTTCTCAAGAATCCTCGCATAGAAGCCAACAGGCTTCGCGAGCTGCCAGATTGCTACAAGGTGA
AGCTGAAAAGTGCTGGATATCGCCTGATTTATCAGGTGATTGATCACGAAATTGTGGTGTTCGTAGTCGCTATCGGAAAG
AGGGAGCGAGAGGCGGCTTATGACACTGCACAGGATCGACTGTCGTCCCAACCCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8835.22 Da
• Isoelectric Point: 4.6017

>AT99468 WP_085732973.1 NZ_CP027561:5557180-5557419 [Pseudomonas fluorescens]
MASINIRIDDELKQRAFAELEKLGLTPSELLRQTLQYVADRGKLPFKAALISEEDEALIAVVTERLAAPQRVKVSLDDL

Nucleotide

• Length: 240 bp

>AT99468 NZ_CP027561:5557180-5557419 [Pseudomonas fluorescens]
ATGGCATCCATCAACATTCGTATCGACGACGAACTGAAACAGCGCGCCTTCGCAGAGCTGGAAAAGCTCGGGCTGACGCC
CTCAGAGCTTCTGCGCCAAACGCTTCAATACGTCGCGGACCGGGGCAAACTACCATTCAAAGCGGCATTGATTAGCGAAG
AAGATGAAGCGCTTATCGCCGTGGTCACTGAACGTCTTGCCGCTCCTCAGCGAGTCAAGGTAAGTCTGGATGACCTATGA