Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 54259..54902 Replicon plasmid unnamed2
Accession NZ_CP027556
Organism Escherichia coli strain 2013C-3513

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag C6991_RS31185 Protein ID WP_001044768.1
Coordinates 54259..54675 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag C6991_RS31190 Protein ID WP_001261287.1
Coordinates 54672..54902 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6991_RS31160 49421..50095 - 675 WP_001341423.1 IS66 family insertion sequence hypothetical protein -
C6991_RS31165 50149..50535 - 387 WP_001066949.1 tyrosine-type recombinase/integrase -
C6991_RS31170 50663..51523 + 861 WP_000704534.1 alpha/beta hydrolase -
C6991_RS31985 52012..52287 + 276 WP_157776129.1 hypothetical protein -
C6991_RS31180 53552..54097 + 546 WP_001165114.1 hypothetical protein -
C6991_RS31185 54259..54675 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
C6991_RS31190 54672..54902 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C6991_RS31210 55462..55848 + 387 WP_106881569.1 hypothetical protein -
C6991_RS31215 55850..56632 + 783 WP_001164205.1 site-specific integrase -
C6991_RS31220 56805..57158 + 354 WP_000864810.1 colicin M immunity protein -
C6991_RS31225 57166..57357 - 192 Protein_61 lipid II-degrading bacteriocin -
C6991_RS31230 57361..57504 - 144 Protein_62 transcriptional regulator -
C6991_RS31235 57571..59010 - 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - toxB / toxB / hlyD / hlyB / hlyA / hlyC / espP 1..91046 91046


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T99460 WP_001044768.1 NZ_CP027556:c54675-54259 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T99460 NZ_CP027556:c54675-54259 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT99460 WP_001261287.1 NZ_CP027556:c54902-54672 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT99460 NZ_CP027556:c54902-54672 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References