Detailed information of TA system TA099455

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4117379..4118033
• Replicon: chromosome
• Accession: NZ_CP027555
• Organism: Escherichia coli strain 2013C-3513

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1EEB2
• Locus tag: C6991_RS22770
• Protein ID: WP_000244777.1
• Coordinates: 4117626..4118033 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: C6991_RS22765
• Protein ID: WP_000354043.1
• Coordinates: 4117379..4117645 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6991_RS22740	4112548..4113291	+	744	WP_000951964.1	SDR family oxidoreductase	-
C6991_RS22745	4113348..4114781	-	1434	WP_001341859.1	6-phospho-beta-glucosidase BglA	-
C6991_RS22750	4114826..4115137	+	312	WP_001182954.1	N(4)-acetylcytidine aminohydrolase	-
C6991_RS22755	4115301..4115960	+	660	WP_000250274.1	hemolysin III family protein	-
C6991_RS22760	4116156..4117136	-	981	WP_000886095.1	tRNA-modifying protein YgfZ	-
C6991_RS22765	4117379..4117645	+	267	WP_000354043.1	FAD assembly factor SdhE	Antitoxin
C6991_RS22770	4117626..4118033	+	408	WP_000244777.1	toxin CptA	Toxin
C6991_RS22775	4118073..4118594	-	522	WP_001055874.1	flavodoxin FldB	-
C6991_RS22780	4118706..4119602	+	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
C6991_RS22785	4119627..4120337	+	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
C6991_RS22790	4120343..4122076	+	1734	WP_000813220.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16031.96 Da
• Isoelectric Point: 11.5202

>T99455 WP_000244777.1 NZ_CP027555:4117626-4118033 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T99455 NZ_CP027555:4117626-4118033 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10563.16 Da
• Isoelectric Point: 5.1599

>AT99455 WP_000354043.1 NZ_CP027555:4117379-4117645 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDLDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT99455 NZ_CP027555:4117379-4117645 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCTGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LFV7