Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2494182..2495014 Replicon chromosome
Accession NZ_CP027555
Organism Escherichia coli strain 2013C-3513

Toxin (Protein)


Gene name yeeV Uniprot ID S1PD64
Locus tag C6991_RS14305 Protein ID WP_000854753.1
Coordinates 2494182..2494556 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID V0ULY5
Locus tag C6991_RS14310 Protein ID WP_001315620.1
Coordinates 2494646..2495014 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6991_RS14260 2489397..2490503 + 1107 WP_001341302.1 N-acetylneuraminate epimerase -
C6991_RS14265 2490568..2491548 + 981 WP_000991415.1 9-O-acetyl-N-acetylneuraminic acid deacetylase -
C6991_RS14270 2491556..2492206 - 651 WP_001037966.1 HNH endonuclease -
C6991_RS32135 2493251..2493403 - 153 WP_001280445.1 hypothetical protein -
C6991_RS14295 2493488..2493685 - 198 WP_000839281.1 DUF957 domain-containing protein -
C6991_RS14300 2493697..2494185 - 489 WP_000777547.1 hypothetical protein -
C6991_RS14305 2494182..2494556 - 375 WP_000854753.1 TA system toxin CbtA family protein Toxin
C6991_RS14310 2494646..2495014 - 369 WP_001315620.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
C6991_RS14315 2495177..2495398 - 222 WP_000692345.1 DUF987 domain-containing protein -
C6991_RS14320 2495461..2495937 - 477 WP_001186774.1 RadC family protein -
C6991_RS14325 2495953..2496438 - 486 WP_000849588.1 antirestriction protein -
C6991_RS14330 2496493..2497310 - 818 Protein_2508 DUF932 domain-containing protein -
C6991_RS14340 2497410..2497643 - 234 WP_001119729.1 DUF905 family protein -
C6991_RS14345 2497722..2498177 - 456 WP_000581504.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14005.00 Da        Isoelectric Point: 8.2905

>T99450 WP_000854753.1 NZ_CP027555:c2494556-2494182 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Download         Length: 375 bp

>T99450 NZ_CP027555:c2494556-2494182 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13474.20 Da        Isoelectric Point: 6.2050

>AT99450 WP_001315620.1 NZ_CP027555:c2495014-2494646 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT99450 NZ_CP027555:c2495014-2494646 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LVU0

Antitoxin

Source ID Structure
AlphaFold DB V0ULY5

References