Detailed information of TA system TA099424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 6408..6838
• Replicon: plasmid unnamed1
• Accession: NZ_CP027554
• Organism: Escherichia coli strain 2013C-3513

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: C6991_RS31405
• Protein ID: WP_001388374.1
• Coordinates: 6674..6838 (+)
• Length: 55 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A6Y0EDY2
• Locus tag: C6991_RS00065
• Protein ID: WP_000079939.1
• Coordinates: 6408..6677 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6991_RS00020	2038..2250	+	213	WP_001301409.1	ANR family transcriptional regulator	-
C6991_RS00025	2495..2956	+	462	WP_000978819.1	thermonuclease family protein	-
C6991_RS00030	3002..3211	+	210	WP_001333231.1	hemolysin expression modulator Hha	-
C6991_RS00035	3249..3839	+	591	WP_000766807.1	DUF2726 domain-containing protein	-
C6991_RS00040	4078..4326	+	249	WP_000083839.1	replication regulatory protein RepA	-
C6991_RS00050	4571..4645	+	75	WP_001375168.1	RepA leader peptide Tap	-
C6991_RS00055	4638..5494	+	857	Protein_10	incFII family plasmid replication initiator RepA	-
C6991_RS31450	6195..6335	+	141	WP_021503378.1	hypothetical protein	-
C6991_RS00065	6408..6677	+	270	WP_000079939.1	hypothetical protein	Antitoxin
C6991_RS31405	6674..6838	+	165	WP_001388374.1	hypothetical protein	Toxin
C6991_RS00070	6886..7809	+	924	WP_001415483.1	hypothetical protein	-
C6991_RS00080	8251..8631	+	381	WP_001696636.1	HTH domain-containing protein	-
C6991_RS00085	8743..9147	+	405	Protein_16	IS91 family transposase	-
C6991_RS00090	9254..10090	-	837	WP_000480972.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
C6991_RS00095	10090..10893	-	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
C6991_RS00100	10954..11768	-	815	Protein_19	sulfonamide-resistant dihydropteroate synthase Sul2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / tet(B)	-	1..70129	70129
	-	flank	IS/Tn	aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B	-	9254..15126	5872

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 55 a.a.
• Molecular weight: 6604.73 Da
• Isoelectric Point: 7.1989

>T99424 WP_001388374.1 NZ_CP027554:6674-6838 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYFGRTHRLTPVLHLYYM

Nucleotide

• Length: 165 bp

>T99424 NZ_CP027554:6674-6838 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAATACATTGCGGAGCAGAACCTGAT
AGCAGCCATTGAACTGGACGAACGGATTGGTTATTTCGGACGAACGCACCGGCTTACGCCGGTTTTACATCTGTACTACA
TGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10233.54 Da
• Isoelectric Point: 6.7263

>AT99424 WP_000079939.1 NZ_CP027554:6408-6677 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT99424 NZ_CP027554:6408-6677 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGTCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCACGGCGATGTACTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGAGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6Y0EDY2