Detailed information of TA system TA099415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4606270..4606895
• Replicon: chromosome
• Accession: NZ_CP027552
• Organism: Escherichia coli strain 2015C-4498

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6953_RS24865
• Protein ID: WP_044805154.1
• Coordinates: 4606270..4606668 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C6953_RS24870
• Protein ID: WP_000450524.1
• Coordinates: 4606668..4606895 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6953_RS24845	4602149..4602349	+	201	WP_000383836.1	YpfN family protein	-
C6953_RS24850	4602459..4603157	-	699	WP_000679812.1	esterase	-
C6953_RS24855	4603231..4605245	-	2015	Protein_4521	tRNA cytosine(34) acetyltransferase TmcA	-
C6953_RS24860	4605260..4606123	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
C6953_RS24865	4606270..4606668	-	399	WP_044805154.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6953_RS24870	4606668..4606895	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6953_RS24875	4607049..4607762	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C6953_RS24880	4607975..4609009	-	1035	WP_106884216.1	outer membrane protein assembly factor BamC	-
C6953_RS24885	4609026..4609904	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C6953_RS24890	4610050..4610622	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C6953_RS24895	4610622..4611092	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14924.28 Da
• Isoelectric Point: 8.5325

>T99415 WP_044805154.1 NZ_CP027552:c4606668-4606270 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVREWFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T99415 NZ_CP027552:c4606668-4606270 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGTGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT99415 WP_000450524.1 NZ_CP027552:c4606895-4606668 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT99415 NZ_CP027552:c4606895-4606668 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33