Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3265744..3266382 Replicon chromosome
Accession NZ_CP027552
Organism Escherichia coli strain 2015C-4498

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag C6953_RS17485 Protein ID WP_000813794.1
Coordinates 3265744..3265920 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C6953_RS17490 Protein ID WP_001270286.1
Coordinates 3265966..3266382 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6953_RS17465 3261363..3262577 - 1215 WP_001307190.1 BenE family transporter YdcO -
C6953_RS17470 3262630..3263166 + 537 WP_106884108.1 DNA-binding transcriptional regulator SutR -
C6953_RS17475 3263239..3265200 + 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C6953_RS17480 3265292..3265522 - 231 WP_000494244.1 YncJ family protein -
C6953_RS17485 3265744..3265920 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C6953_RS17490 3265966..3266382 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C6953_RS17495 3266461..3267867 + 1407 WP_044805955.1 PLP-dependent aminotransferase family protein -
C6953_RS17500 3268112..3269257 + 1146 WP_044805956.1 ABC transporter substrate-binding protein -
C6953_RS17505 3269275..3270288 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
C6953_RS17510 3270289..3271230 + 942 WP_106884109.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3260175..3261323 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T99403 WP_000813794.1 NZ_CP027552:3265744-3265920 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T99403 NZ_CP027552:3265744-3265920 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT99403 WP_001270286.1 NZ_CP027552:3265966-3266382 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT99403 NZ_CP027552:3265966-3266382 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References