Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2861608..2862079 Replicon chromosome
Accession NZ_CP027552
Organism Escherichia coli strain 2015C-4498

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag C6953_RS15135 Protein ID WP_001303511.1
Coordinates 2861608..2861886 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C6953_RS15140 Protein ID WP_001302048.1
Coordinates 2861888..2862079 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6953_RS15095 2856826..2857095 - 270 WP_000003742.1 excisionase -
C6953_RS15100 2857157..2859628 - 2472 WP_106878764.1 exonuclease -
C6953_RS15105 2859721..2859912 - 192 WP_001090200.1 DUF1482 family protein -
C6953_RS15110 2859909..2860097 - 189 WP_000449192.1 cell division inhibition protein DicB -
C6953_RS15120 2860666..2860884 - 219 WP_001171930.1 hypothetical protein -
C6953_RS15125 2860956..2861255 - 300 WP_001240334.1 hypothetical protein -
C6953_RS15135 2861608..2861886 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6953_RS15140 2861888..2862079 - 192 WP_001302048.1 hypothetical protein Antitoxin
C6953_RS15145 2862100..2862471 - 372 WP_001169687.1 hypothetical protein -
C6953_RS15150 2862569..2862871 + 303 WP_106884078.1 transcriptional regulator -
C6953_RS15155 2862868..2863293 + 426 WP_000693943.1 Rha family transcriptional regulator -
C6953_RS15160 2863316..2864278 + 963 WP_000095671.1 helix-turn-helix domain-containing protein -
C6953_RS15165 2864319..2864741 + 423 WP_001151120.1 DUF977 family protein -
C6953_RS15170 2864738..2864992 + 255 WP_000004322.1 hypothetical protein -
C6953_RS15175 2864985..2865296 + 312 WP_001002672.1 hypothetical protein -
C6953_RS15180 2865602..2866180 + 579 WP_001204666.1 sce7726 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - espX7/nleL / espN / espK 2851471..2931436 79965


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T99402 WP_001303511.1 NZ_CP027552:c2861886-2861608 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T99402 NZ_CP027552:c2861886-2861608 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT99402 WP_001302048.1 NZ_CP027552:c2862079-2861888 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT99402 NZ_CP027552:c2862079-2861888 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References