Detailed information of TA system TA099396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 1267045..1267640
• Replicon: chromosome
• Accession: NZ_CP027552
• Organism: Escherichia coli strain 2015C-4498

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: C6953_RS06670
• Protein ID: WP_044805826.1
• Coordinates: 1267290..1267640 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: U9Y2K1
• Locus tag: C6953_RS06665
• Protein ID: WP_001223208.1
• Coordinates: 1267045..1267296 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6953_RS06655	1262710..1266489	+	3780	WP_106883961.1	autotransporter assembly complex protein TamB	-
C6953_RS06660	1266492..1266833	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
C6953_RS06665	1267045..1267296	+	252	WP_001223208.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
C6953_RS06670	1267290..1267640	+	351	WP_044805826.1	endoribonuclease toxin ChpB	Toxin
C6953_RS06675	1267720..1268250	-	531	WP_044805825.1	inorganic diphosphatase	-
C6953_RS06680	1268560..1269516	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
C6953_RS06685	1269656..1271158	+	1503	WP_106883962.1	sugar ABC transporter ATP-binding protein	-
C6953_RS06690	1271172..1272194	+	1023	WP_001296689.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-115)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12479.39 Da
• Isoelectric Point: 5.6219

>T99396 WP_044805826.1 NZ_CP027552:1267290-1267640 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMVDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T99396 NZ_CP027552:1267290-1267640 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGGTGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.58 Da
• Isoelectric Point: 4.4201

>AT99396 WP_001223208.1 NZ_CP027552:1267045-1267296 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT99396 NZ_CP027552:1267045-1267296 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LQ26