Detailed information of TA system TA099389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 118044..118804
• Replicon: plasmid unnamed
• Accession: NZ_CP027551
• Organism: Escherichia coli strain 2015C-4136CT1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8H9BF00
• Locus tag: C6950_RS26160
• Protein ID: WP_062859951.1
• Coordinates: 118044..118532 (-)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: C6950_RS26165
• Protein ID: WP_073534343.1
• Coordinates: 118520..118804 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6950_RS26135	114619..115479	+	861	WP_062859948.1	alpha/beta hydrolase	-
C6950_RS26150	116438..117061	-	624	WP_062859950.1	proQ/FINO family protein	-
C6950_RS26155	117121..117531	-	411	Protein_143	serine acetyltransferase	-
C6950_RS26160	118044..118532	-	489	WP_062859951.1	GNAT family N-acetyltransferase	Toxin
C6950_RS26165	118520..118804	-	285	WP_073534343.1	DUF1778 domain-containing protein	Antitoxin
C6950_RS26180	119723..120745	-	1023	WP_096169364.1	replication initiation protein	-
C6950_RS26185	120738..120812	-	75	WP_064716759.1	RepA leader peptide Tap	-
C6950_RS26195	121053..121307	-	255	WP_000083830.1	replication regulatory protein RepA	-
C6950_RS26200	121547..122137	-	591	WP_000766816.1	DUF2726 domain-containing protein	-
C6950_RS26205	122176..122385	-	210	WP_001299729.1	hemolysin expression modulator Hha	-
C6950_RS26210	122431..122892	-	462	WP_000978818.1	thermonuclease family protein	-
C6950_RS26215	123138..123350	-	213	WP_001299730.1	ANR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	bfpK / bfpJ / bfpI / bfpH / bfpP / bfpF / bfpE / bfpD / bfpB / bfpG / bfpA	1..162810	162810
	-	flank	IS/Tn	-	-	113360..113680	320

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (12-88)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17614.34 Da
• Isoelectric Point: 9.8566

>T99389 WP_062859951.1 NZ_CP027551:c118532-118044 [Escherichia coli]
MGGVTAPEPLSSFHQAAEFVSGEAVLDDWLKQKGLKNQALGATRTFVVCRKGSQQIVGFYSLATGSVNHTEAAGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVCRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKSQEQTLFLKLP
GK

Nucleotide

• Length: 489 bp

>T99389 NZ_CP027551:c118532-118044 [Escherichia coli]
ATGGGAGGTGTAACAGCACCAGAACCTTTGTCCTCATTTCATCAGGCCGCTGAATTTGTCAGTGGTGAAGCTGTGCTTGA
TGACTGGTTAAAGCAGAAGGGGCTGAAAAATCAGGCTCTCGGAGCCACACGGACTTTTGTTGTATGCAGAAAAGGTTCGC
AACAAATAGTCGGTTTTTACTCTCTGGCCACCGGGAGTGTTAATCATACAGAGGCTGCAGGCAACCTTCGGCGCAATATG
CCAGACCCTATACCTGTTATTATACTTGCACGCCTGGCCGTTGATGTGTCATTCCGTGGAAAAGGGCTTGGCGCAGATTT
ACTTCATGATGCTGTTTGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAAGCCAGGAGCAAACATTGTTTCTTAAACTCCCC
GGCAAGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10947.62 Da
• Isoelectric Point: 4.7037

>AT99389 WP_073534343.1 NZ_CP027551:c118804-118520 [Escherichia coli]
MIYGGFMKSGVQLNLRARESQRILIDTAAEILHKSRTDFILEMACKAAEDIILDRRVFNFNDRQYEEFIEMLDAPVADET
AIEKLLARKPQWEV

Nucleotide

• Length: 285 bp

>AT99389 NZ_CP027551:c118804-118520 [Escherichia coli]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGGGCAAGAGAATCTCAGCGGATACTCATTGATAC
CGCTGCAGAAATTCTTCATAAGTCGCGTACAGACTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATATTATTCTTG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATCGAAATGCTTGATGCTCCGGTTGCTGATGAGACT
GCCATCGAAAAACTGCTGGCGAGGAAACCTCAATGGGAGGTGTAA