Detailed information of TA system TA099388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 84655..85180
• Replicon: plasmid unnamed
• Accession: NZ_CP027551
• Organism: Escherichia coli strain 2015C-4136CT1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: C6950_RS25945
• Protein ID: WP_001159871.1
• Coordinates: 84655..84960 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: C6950_RS25950
• Protein ID: WP_062860119.1
• Coordinates: 84962..85180 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6950_RS25900	79991..81012	+	1022	Protein_98	alpha-galactosidase	-
C6950_RS25905	81075..81188	+	114	Protein_99	MFS transporter	-
C6950_RS25910	81183..81482	-	300	Protein_100	integrase core domain-containing protein	-
C6950_RS25915	81481..81936	+	456	Protein_101	IS3 family transposase	-
C6950_RS25920	81969..82511	+	543	Protein_102	IS3-like element ISEc48 family transposase	-
C6950_RS26940	82699..83262	+	564	WP_062860118.1	hypothetical protein	-
C6950_RS25930	83517..84170	-	654	Protein_104	IS3 family transposase	-
C6950_RS26945	84167..84352	+	186	Protein_105	IS91 family transposase	-
C6950_RS25940	84522..84654	-	133	Protein_106	phage integrase family protein	-
C6950_RS25945	84655..84960	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
C6950_RS25950	84962..85180	-	219	WP_062860119.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
C6950_RS25960	85776..86020	+	245	Protein_109	hypothetical protein	-
C6950_RS25965	86020..86610	+	591	WP_062860120.1	hypothetical protein	-
C6950_RS26950	86735..87598	-	864	WP_062860121.1	IS3 family transposase	-
C6950_RS26955	87574..88080	-	507	WP_062897179.1	helix-turn-helix domain-containing protein	-
C6950_RS25975	88385..89200	+	816	WP_062860059.1	helix-turn-helix transcriptional regulator	-
C6950_RS25980	89440..89577	-	138	WP_062860058.1	hypothetical protein	-
C6950_RS25985	89588..89719	-	132	WP_062860057.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	bfpK / bfpJ / bfpI / bfpH / bfpP / bfpF / bfpE / bfpD / bfpB / bfpG / bfpA	1..162810	162810

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T99388 WP_001159871.1 NZ_CP027551:c84960-84655 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T99388 NZ_CP027551:c84960-84655 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8402.37 Da
• Isoelectric Point: 4.5203

>AT99388 WP_062860119.1 NZ_CP027551:c85180-84962 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVAWFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT99388 NZ_CP027551:c85180-84962 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCTGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A829CCE8