Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4776290..4776908 Replicon chromosome
Accession NZ_CP027550
Organism Escherichia coli strain 2015C-4136CT1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C6950_RS25060 Protein ID WP_001291435.1
Coordinates 4776290..4776508 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C6950_RS25065 Protein ID WP_000344800.1
Coordinates 4776534..4776908 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6950_RS25030 4772180..4772491 - 312 WP_000409908.1 MGMT family protein -
C6950_RS25040 4772870..4773223 + 354 WP_000878140.1 DUF1428 domain-containing protein -
C6950_RS25045 4773265..4774815 - 1551 WP_001357014.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C6950_RS25050 4774979..4775449 - 471 WP_000136192.1 YlaC family protein -
C6950_RS25055 4775565..4776116 - 552 WP_000102539.1 maltose O-acetyltransferase -
C6950_RS25060 4776290..4776508 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C6950_RS25065 4776534..4776908 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C6950_RS25070 4777453..4780602 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
C6950_RS25075 4780625..4781818 - 1194 WP_001374568.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4770781..4772154 1373


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T99387 WP_001291435.1 NZ_CP027550:c4776508-4776290 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T99387 NZ_CP027550:c4776508-4776290 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT99387 WP_000344800.1 NZ_CP027550:c4776908-4776534 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT99387 NZ_CP027550:c4776908-4776534 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References