Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4336825..4337375 Replicon chromosome
Accession NZ_CP027550
Organism Escherichia coli strain 2015C-4136CT1

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag C6950_RS22805 Protein ID WP_001160966.1
Coordinates 4337061..4337375 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag C6950_RS22800 Protein ID WP_000125566.1
Coordinates 4336825..4337058 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6950_RS22775 4331898..4332185 + 288 WP_000203741.1 ferredoxin-like protein FixX -
C6950_RS22780 4332244..4333575 + 1332 WP_001183207.1 MFS transporter -
C6950_RS22785 4333683..4334213 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
C6950_RS22790 4334206..4336068 + 1863 WP_001356883.1 glutathione-regulated potassium-efflux system protein KefC -
C6950_RS22795 4336260..4336739 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
C6950_RS22800 4336825..4337058 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
C6950_RS22805 4337061..4337375 + 315 WP_001160966.1 CcdB family protein Toxin
C6950_RS22810 4337372..4338220 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
C6950_RS22815 4338227..4338604 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
C6950_RS22820 4338607..4339428 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
C6950_RS22825 4339425..4340414 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
C6950_RS22830 4340414..4341700 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T99386 WP_001160966.1 NZ_CP027550:4337061-4337375 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T99386 NZ_CP027550:4337061-4337375 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT99386 WP_000125566.1 NZ_CP027550:4336825-4337058 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT99386 NZ_CP027550:4336825-4337058 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References