Detailed information of TA system TA099385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4227515..4228023
• Replicon: chromosome
• Accession: NZ_CP027550
• Organism: Escherichia coli strain 2015C-4136CT1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: C6950_RS22245
• Protein ID: WP_001534843.1
• Coordinates: 4227515..4227724 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0V9L4L3
• Locus tag: C6950_RS22250
• Protein ID: WP_000063161.1
• Coordinates: 4227721..4228023 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6950_RS22210	4223221..4224141	+	921	WP_001357522.1	Rpn family recombination-promoting nuclease/putative transposase	-
C6950_RS22215	4224124..4224447	-	324	WP_021560186.1	winged helix-turn-helix domain-containing protein	-
C6950_RS22220	4224625..4224789	+	165	WP_000394261.1	DUF1127 domain-containing protein	-
C6950_RS22225	4224831..4225158	-	328	Protein_4077	endoribonuclease SymE	-
C6950_RS22230	4225373..4226041	-	669	Protein_4078	DUF3387 domain-containing protein	-
C6950_RS22235	4226035..4226208	-	174	Protein_4079	SAM-dependent DNA methyltransferase	-
C6950_RS22240	4226253..4227419	-	1167	WP_000800830.1	restriction endonuclease	-
C6950_RS22245	4227515..4227724	+	210	WP_001534843.1	BrnT family toxin	Toxin
C6950_RS22250	4227721..4228023	+	303	WP_000063161.1	BrnA antitoxin family protein	Antitoxin
C6950_RS22255	4228057..4229013	-	957	WP_001600951.1	GTPase	-
C6950_RS22260	4229024..4229227	-	204	WP_000467859.1	YbdD/YjiX family protein	-
C6950_RS22265	4229345..4231495	-	2151	WP_001314407.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T99385 WP_001534843.1 NZ_CP027550:4227515-4227724 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T99385 NZ_CP027550:4227515-4227724 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.10 Da
• Isoelectric Point: 9.4646

>AT99385 WP_000063161.1 NZ_CP027550:4227721-4228023 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT99385 NZ_CP027550:4227721-4228023 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGTGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0V9L4L3