Detailed information of TA system TA099379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3050251..3051005
• Replicon: chromosome
• Accession: NZ_CP027550
• Organism: Escherichia coli strain 2015C-4136CT1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A822TTG1
• Locus tag: C6950_RS16155
• Protein ID: WP_021560068.1
• Coordinates: 3050520..3051005 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: C6950_RS16150
• Protein ID: WP_000801915.1
• Coordinates: 3050251..3050529 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6950_RS16145	3047479..3050184	+	2706	WP_000906938.1	HTH-type transcriptional regulator MalT	-
C6950_RS16150	3050251..3050529	+	279	WP_000801915.1	DUF1778 domain-containing protein	Antitoxin
C6950_RS16155	3050520..3051005	+	486	WP_021560068.1	GNAT family N-acetyltransferase	Toxin
C6950_RS16160	3051055..3052083	-	1029	WP_001356914.1	RNA 3'-terminal phosphate cyclase	-
C6950_RS16165	3052087..3053313	-	1227	WP_001105471.1	RNA-splicing ligase RtcB	-
C6950_RS16170	3053532..3054146	+	615	Protein_2971	DNA-binding transcriptional regulator RtcR	-
C6950_RS16175	3054128..3054886	-	759	WP_001296480.1	DeoR/GlpR family transcriptional regulator	-
C6950_RS16180	3054903..3055733	-	831	WP_000928734.1	rhomboid family intramembrane serine protease GlpG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3041765..3067280	25515

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17680.60 Da
• Isoelectric Point: 9.4066

>T99379 WP_021560068.1 NZ_CP027550:3050520-3051005 [Escherichia coli]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSSLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T99379 NZ_CP027550:3050520-3051005 [Escherichia coli]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCAGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGCTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGGGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10450.97 Da
• Isoelectric Point: 4.6336

>AT99379 WP_000801915.1 NZ_CP027550:3050251-3050529 [Escherichia coli]
MKPESKETPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT99379 NZ_CP027550:3050251-3050529 [Escherichia coli]
ATGAAACCGGAAAGCAAAGAAACACCAATCAATATCAGGGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTGGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Toxin

• AlphaFold DB: A0A822TTG1