Detailed information of TA system TA099362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 86162..86697
• Replicon: plasmid unnamed
• Accession: NZ_CP027549
• Organism: Escherichia coli strain 2014C-3061

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C6992_RS28935
• Protein ID: WP_001270421.1
• Coordinates: 86410..86697 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1V0M724
• Locus tag: C6992_RS28930
• Protein ID: WP_001132893.1
• Coordinates: 86162..86413 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6992_RS28900	81388..82584	+	1197	WP_033815853.1	IS91 family transposase	-
C6992_RS28905	82772..83140	+	369	Protein_91	DUF4113 domain-containing protein	-
C6992_RS28910	83280..84242	-	963	WP_000756329.1	ParB/RepB/Spo0J family partition protein	-
C6992_RS28915	84239..85444	-	1206	WP_000817642.1	AAA family ATPase	-
C6992_RS29605	85818..85988	+	171	WP_000402943.1	hypothetical protein	-
C6992_RS28930	86162..86413	+	252	WP_001132893.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C6992_RS28935	86410..86697	+	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6992_RS29610	86699..86821	+	123	Protein_97	IS3 family transposase	-
C6992_RS28940	86907..87272	+	366	WP_000091308.1	hypothetical protein	-
C6992_RS28945	87272..88468	+	1197	WP_033815853.1	IS91 family transposase	-
C6992_RS28950	88656..89024	+	369	Protein_100	DUF4113 domain-containing protein	-
C6992_RS28955	89164..90126	-	963	WP_000756329.1	ParB/RepB/Spo0J family partition protein	-
C6992_RS28960	90123..91328	-	1206	WP_000817642.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	stcE / exeE / exeG / hlyD / hlyB / hlyA / hlyC	1..94116	94116
	-	inside	IScluster/Tn	-	-	80415..93662	13247

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T99362 WP_001270421.1 NZ_CP027549:86410-86697 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T99362 NZ_CP027549:86410-86697 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAG
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9192.50 Da
• Isoelectric Point: 4.2108

>AT99362 WP_001132893.1 NZ_CP027549:86162-86413 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPIAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT99362 NZ_CP027549:86162-86413 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGATCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A1V0M724