Detailed information of TA system TA099361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 3813..4348
• Replicon: plasmid unnamed
• Accession: NZ_CP027549
• Organism: Escherichia coli strain 2014C-3061

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C6992_RS28430
• Protein ID: WP_001270421.1
• Coordinates: 4061..4348 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1V0M724
• Locus tag: C6992_RS28425
• Protein ID: WP_001132893.1
• Coordinates: 3813..4064 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6992_RS28400	423..791	+	369	Protein_1	DUF4113 domain-containing protein	-
C6992_RS28405	931..1893	-	963	WP_000756329.1	ParB/RepB/Spo0J family partition protein	-
C6992_RS28410	1890..3095	-	1206	WP_000817642.1	AAA family ATPase	-
C6992_RS29585	3469..3639	+	171	WP_000402943.1	hypothetical protein	-
C6992_RS28425	3813..4064	+	252	WP_001132893.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C6992_RS28430	4061..4348	+	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6992_RS29590	4350..4472	+	123	Protein_7	IS3 family transposase	-
C6992_RS28435	4558..4923	+	366	WP_000091308.1	hypothetical protein	-
C6992_RS28440	4923..6119	+	1197	WP_033815853.1	IS91 family transposase	-
C6992_RS28445	6308..6460	-	153	Protein_10	transposase	-
C6992_RS28455	7934..8755	+	822	WP_001302199.1	polysaccharide deacetylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	stcE / exeE / exeG / hlyD / hlyB / hlyA / hlyC	1..94116	94116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T99361 WP_001270421.1 NZ_CP027549:4061-4348 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T99361 NZ_CP027549:4061-4348 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAG
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9192.50 Da
• Isoelectric Point: 4.2108

>AT99361 WP_001132893.1 NZ_CP027549:3813-4064 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPIAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT99361 NZ_CP027549:3813-4064 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGATCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A1V0M724