Detailed information of TA system TA099360

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5113939..5114776
• Replicon: chromosome
• Accession: NZ_CP027548
• Organism: Escherichia coli strain 2014C-3061

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9LL67
• Locus tag: C6992_RS27335
• Protein ID: WP_000854687.1
• Coordinates: 5114396..5114776 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: C6992_RS27330
• Protein ID: WP_012816826.1
• Coordinates: 5113939..5114307 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6992_RS27290	5110126..5110803	+	678	WP_024226808.1	hypothetical protein	-
C6992_RS27295	5110819..5111229	+	411	WP_000848829.1	hypothetical protein	-
C6992_RS27305	5111449..5112270	+	822	WP_001234566.1	DUF945 domain-containing protein	-
C6992_RS27310	5112270..5112515	+	246	WP_000680588.1	hypothetical protein	-
C6992_RS27315	5112609..5113082	+	474	WP_001364892.1	antirestriction protein	-
C6992_RS27320	5113098..5113574	+	477	WP_001364966.1	RadC family protein	-
C6992_RS27325	5113643..5113864	+	222	WP_000691817.1	DUF987 domain-containing protein	-
C6992_RS27330	5113939..5114307	+	369	WP_012816826.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6992_RS27335	5114396..5114776	+	381	WP_000854687.1	TA system toxin CbtA family protein	Toxin
C6992_RS27340	5114773..5115261	+	489	WP_032314654.1	hypothetical protein	-
C6992_RS27345	5115281..5115478	+	198	WP_000772026.1	DUF957 domain-containing protein	-
C6992_RS27350	5115563..5116408	+	846	WP_106904405.1	DUF4942 domain-containing protein	-
C6992_RS27360	5116696..5117202	+	507	WP_000228940.1	G/U mismatch-specific DNA glycosylase	-
C6992_RS27365	5117280..5119121	-	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14199.13 Da
• Isoelectric Point: 6.8615

>T99360 WP_000854687.1 NZ_CP027548:5114396-5114776 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T99360 NZ_CP027548:5114396-5114776 [Escherichia coli]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13780.56 Da
• Isoelectric Point: 6.4763

>AT99360 WP_012816826.1 NZ_CP027548:5113939-5114307 [Escherichia coli]
VSDTLHETNYPNDNHNRPWWGLPCTVTPCFGARLVQEGNRLHYLDDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVALYAKGLTCDADTLGSCGYVYLAVYPTPDMKK

Nucleotide

• Length: 369 bp

>AT99360 NZ_CP027548:5113939-5114307 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACAACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGACGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGGCACTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LL67