Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) hicAB/-
Location 4668532..4669163 Replicon chromosome
Accession NZ_CP027548
Organism Escherichia coli strain 2014C-3061

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag C6992_RS24820 Protein ID WP_001259385.1
Coordinates 4668532..4668807 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C6992_RS24825 Protein ID WP_000593555.1
Coordinates 4668804..4669163 (+) Length 120 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6992_RS24810 4664574..4667309 + 2736 WP_032314191.1 ribosome-associated ATPase/putative transporter RbbA -
C6992_RS24815 4667309..4668433 + 1125 WP_001216257.1 ABC transporter permease -
C6992_RS24820 4668532..4668807 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
C6992_RS24825 4668804..4669163 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6992_RS24830 4669283..4669684 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
C6992_RS24835 4669690..4670496 - 807 WP_000173711.1 nickel import ATP-binding protein NikE -
C6992_RS24840 4670493..4671257 - 765 WP_001136215.1 nickel import ATP-binding protein NikD -
C6992_RS24845 4671257..4672090 - 834 WP_001008966.1 nickel ABC transporter permease subunit NikC -
C6992_RS24850 4672087..4673031 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4661522..4669684 8162


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(30-80)

Antitoxin

(60-112)


Sequences


Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T99357 WP_001259385.1 NZ_CP027548:4668532-4668807 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T99357 NZ_CP027548:4668532-4668807 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT99357 WP_000593555.1 NZ_CP027548:4668804-4669163 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT99357 NZ_CP027548:4668804-4669163 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References