Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 3812269..3812864 Replicon chromosome
Accession NZ_CP027548
Organism Escherichia coli strain 2014C-3061

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag C6992_RS20540 Protein ID WP_000239579.1
Coordinates 3812269..3812619 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID A0A240D6C8
Locus tag C6992_RS20545 Protein ID WP_001223212.1
Coordinates 3812613..3812864 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6992_RS20520 3807715..3808737 - 1023 WP_001353963.1 ABC transporter permease -
C6992_RS20525 3808751..3810253 - 1503 WP_000210553.1 sugar ABC transporter ATP-binding protein -
C6992_RS20530 3810393..3811349 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
C6992_RS20535 3811659..3812189 + 531 WP_000055072.1 inorganic diphosphatase -
C6992_RS20540 3812269..3812619 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
C6992_RS20545 3812613..3812864 - 252 WP_001223212.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
C6992_RS20550 3813076..3813417 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
C6992_RS20555 3813420..3817199 - 3780 WP_000060920.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

Antitoxin PemI-like

(1-83)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T99355 WP_000239579.1 NZ_CP027548:c3812619-3812269 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T99355 NZ_CP027548:c3812619-3812269 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTTGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9301.61 Da        Isoelectric Point: 4.6509

>AT99355 WP_001223212.1 NZ_CP027548:c3812864-3812613 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT99355 NZ_CP027548:c3812864-3812613 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A240D6C8

References